Mycoplasma haemofelis (Mhf), Candidatus Mycoplasma haemominutum (CMhm), and Candidatus Mycoplasma turicensis (CMt) are the major feline hemotropic Mycoplasma (FHM) species identified in cats worldwide. Data concerning FHM in Egypt is limited; therefore, the current study aimed to gain further insights into disease epidemiology by investigating FHM molecular prevalence, risk factors, and hemogram abnormalities in 246 Egyptian cats (shelter-housed and client-owned) during 2022–2024. Additionally, 16 S rRNA gene-based maximum-likelihood (ML) phylogenetic analysis was performed for all CMt-positive samples for the first time in Egypt. FHM was detected in 16.3% (n = 40) of cats and typed as CMhm, Mhf, and CMt in 15.4% (n = 38), 3.6% (n = 9), and 2.4% (n = 6) of cats, respectively. Single CMhm and Mhf infections were detected in 12.6% (n = 31) and 0.8% (n = 2) of cats …

Infectious blood diseases are the most detrimental to the health of buffaloes and camels, and they represent the main obstacle to productivity. In Qena Governorate, there is little information about infectious blood diseases, despite their financial losses. Thus, the aim of this investigation was to study the current situation of infectious blood diseases among buffaloes and camels in Qena Governorate. Animals used in this study were obtained from the cities of Nag Hammadi and Farshut in Qena Governorate of Egypt. Whole blood and serum samples were collected for laboratory investigation using Giemsa-stained blood smears and enzyme-linked immunosorbent assay (ELISA), respectively. The clinical findings accompanied by infectious blood diseases in examined animals were fully discussed. Two (2.25%) and 9 (10.11%) of 89 examined buffaloes were positive for merozoites of Babesia spp. and piroplasm of Theileria spp., respectively, while 1 (1.10%) of 91 investigated camels was positive for Trypanosoma evansi by using Giemsa-stained blood smears.  Anaplasma spp. antibodies were detected in 62 (69.66%) of 89 examined buffaloes, and 1 (1.10%) of 91 studied camels was serologically positive for Theileria spp. antigen. The study thoroughly covered some risk factors associated with infectious blood diseases in examined animals, including age, sex, type of animal breeding, seasonal variation, and locality. To reduce the occurrence of infectious blood diseases, it is advised to emphasize the need for implementing efficient prevention and control methods throughout Egypt.

Introduction: Fascioliasis, a significant global zoonotic disease caused by trematode parasites of the genus Fasciola, affects various livestock species. Aim: This study aimed to identify demographic, epidemiological, clinical manifestations, pathological, and genetic characteristics in New Valley, Egypt’s human, and cattle populations.

Methods: This study is made of two parts, the first part is a cohort study of 1000 cattle slaughtered at three abattoirs in El Kharja, El Dakhilah, and El Farafra from February 2023 to January 2024. A retrospective analysis of patients visiting El Kharja hospital with clinical symptoms and confirmed with coprological and radiological examinations.

Results: The study revealed a high prevalence in cattle (23%) and humans (3.6%). Enrolled human fascioliasis was diagnosed in 58.3% and 41.7% by coprological and radiological analysis, respectively. A 66.7% of enrolled cases were females, with a median age range of 37 ± 13 years old. Treatment outcomes demonstrated a response of 25%, 50%, and 25% to single, two, and three doses of the treatment, respectively. In cattle, infection rate was 20.0% in females compared to 24.9% in males with low prevalence in animals <1 year (12.7%) and high in animals >3 years (30.4%). Autumn had the highest prevalence (28.7%), whereas summer had the lowest prevalence (18.0%). A significant difference in the prevalence of fasciolasis was observed between human and animals. Morphological and histopathological analysis elucidated acute and chronic manifestations of hepatic fascioliasis with ectopic migration to cattle lung tissue. Genetic characterization of ectopic worm confirmed Fasciola hepatica infection, with genetic similarity to human isolates from Iran.

Discussion: The study highlights the importance of one health approach in understanding and managing fascioliasis.