This study evaluated the effects of dietary lignan supplementation on the performance, antioxidant status, immune response, and meat composition of Japanese quails (Coturnix coturnix japonica). A total of 105 one-day-old unsexed Japanese quails were randomly assigned one of five treatment groups, each with 3 replicates of 7 chicks. The groups consisted of a control group that fed a basal diet (BD), an antibiotic group (0.05% bacitracin methylene salicylate in BD), and three lignan-supplemented groups (50, 100, and 150 mg lignans/kg diet, designated L50, L100, and L150, respectively). Dietary lignans had no significant effect on growth performance parameters, including body weight, weight gain, or feed conversion ratio. However, the L100 group exhibited significant reductions in serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), creatinine, and uric acid (P<0.05) compared to other groups. Furthermore, lignan supplementation significantly lowered serum cholesterol and total lipids (P<0.05), without affecting triglycerides or glucose. In the L100 group, a significant increase in antioxidant markers, including glutathione peroxidase (GSH-Px), total antioxidant capacity (T-AOC), and total superoxide dismutase (T-SOD), was observed, alongside a significant reduction in malondialdehyde (MDA) levels (P<0.05). Regarding inflammatory cytokines, both the L100 and L150 groups showed significantly reduced levels of interleukin-1 (IL-1β) and TNF (P<0.05), while lower levels of interleukin-6 (IL-6) and interleukin-12 (IL-12) were observed in Anti group followed by L100 group (P<0.05). Lower levels of triglyceride, cholesterol and malondialdehyde (TBARS) in meat were observed in L150 group (P<0.05). In conclusion, the addition of lignans in the diet of Japanese quails did not affect the quail performance, while level of 100 lignans mg/kg diet improved the biochemical, antioxidant system, and immune activities.